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3 www.lgcgenomics.com KASP - User guide and manual 1. Introduction The Kompetitive Allele Specific PCR genotyping system (KASPTM ) is a homogeneous, fluorescent, endpoint genotyping technology. KASP offers the simplest, most cost-effective and flexible way to determine both SNP and insertion/deletion genotypes. Analysis can be carried out in 96-, 384- and 1536-well plate formats. The KASP genotyping system is comprised of two components: 1. The SNP specific KASP Assay mix, separately purchased as a KASP by Design (KBD) or KASP on Demand (KOD) assay (http://www.lgcgenomics.com/genotyping/kasp-genotyping-reagents/kasp-assays -kbd-kod/) 2.
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Despite the fact that KASPar genotyping assays have come to the market very recently, they have started to be used for a large number of commercial species. In maize, a set of 695 highly polymorphic gene-based SNPs from a total of 13,882 GG-validated SNPs were selected and converted into KASPar genotyping assay with a success rate of 98% 43. The KASP chemistry functions equally well in 96-, 384-, and 1,536-well microtiter plate formats and has been utilized over many years in large and small laboratories by users across the fields of. Jan 24, 2018 They were run on a StepOne real‐time PCR machine (Applied Biosystems) on the DNA samples used to generate the library. PCR was carried out in 8 μl single‐locus reactions following thermal cycling conditions recommended in the KASP user guide (LGC Genomics, 2013). The quality of the genotyping cluster plots was visually assessed. Genotyping System Reagent Manual. The KASPar SNP genotyping chemistry allows the user to design and develop genotyping assays. 1.8mM as some assays must be run at this concentration (see troubleshooting guide), therefore for most assays, MgCl.
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User Guide Definition
The universal KASP Master mix, which contains all other required components including the universal fluorescent reporting system and a specially developed Taq polymerase (http://www.lgcgenomics.com/genotyping/kasp-genotyping-reagents/). The KASP genotyping system has been used successfully in a wide variety of organisms, achieving well over 90% SNP to assay conversion rate.
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Kasp Genotyping Chemistry User Guide And Manual Download
2. Principal of the KASP genotyping assay Refer to the website for full animation. http://www.lgcgenomics.com/genotyping/kasp-genotyping-reagents/how-does-kas p-work/ KASP Assay mix KASP Master mix Sample DNA KASP genotyping reaction
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